Hemozoin Enhances Maturation of Murine Bone Marrow Derived Macrophages and Myeloid Dendritic Cells

Authors

Kashif-Ur-Rehman - Main Clinical Pathology Laboratories, Mayo Hospital, Lahore, Pakistan

Ramesh Kumar Department of Health System Policy, Health Services Academy, Islamabad, Pakistan

Shahid Waseem Department of Biochemistry, Quaid-i-Azam University, Islamabad, Pakistan | Department of Immunology, Stockholm University, Stockholm, Sweden

Tariq Mahmood Nano Science and Catalysis Division, National Centre for Physics, Islamabad, Pakistan

Abstract:

Background: Falciparum malaria is a severe health burden worldwide. Antigen presenting cells are reported to be affected by erythrocytic stage of the parasite. Malarial hemozoin (HZ), a metabolite of malaria parasite, has adjuvant properties and may play a role in the induction of immune response against the parasite. Objective: To determine the immunological impact of hemozoin on the capacity of innate immune cells maturation. Methods: Plasmodium falciparum (F32 strain) was cultured in O+ blood group up to 18% parasitemia. Natural hemozoin was extracted from infected red blood cells. Murine bone marrow derived macrophages and myeloid dendritic cells were stimulated with 4 ߤg/mL or 40 ߤg/mL of synthetic hemozoin (β-hematin) or natural hemozoin. We assessed the immunomodulatory role of synthetic or natural hemozoin in vitro by flowcytometric analysis. Results: The maturation markers MHCII, CD80 and CD86 were significantly upregulated (p

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